Michael Eisenstein describes the current toolbox for studying extracellular vesicles. He looks at the state of the art in terms of methods for isolating and characterizing EVs from biological samples, as well as imaging strategies and other techniques for dissecting EV-mediated intercellular communication both in vitro and in vivo. The Tech Feature looks at the various technical challenges that remain to be overcome, including ongoing efforts to improve the robustness and reproducibility of EV research. 

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